📞 Call Now 💬 WhatsApp
🏛️ ART Act 2021 Certified
📋 TGMC Reg: 50624
4.7★ Google Rated
🏆 19+ Years Experience
👨‍👩‍👧 10,000+ Families
🌍 NRI Patients Welcome
💉 Needleless IVF Pioneer
🤰 Safe Delivery Center

IVF Timeline Day by Day

A complete IVF calendar — exactly what happens on every day of your IVF cycle, from your first injection through to your pregnancy test. Knowing the schedule removes uncertainty and helps you plan your work, family, and travel around treatment. Mother Hospitals & IVF Center, Boduppal, Hyderabad.

Quick Answer: An IVF cycle takes 28–33 days from first injection to pregnancy test. Stimulation injections: Days 2–13 (10–14 days). Egg retrieval: Day 14. Embryo culture: Days 14–19. Embryo transfer: Day 17–19. Pregnancy test (beta-hCG blood test): Day 31–33 (14 days after transfer). Pre-cycle tests add 1–2 weeks before this.
28–33
Days per IVF Cycle
6–9
Clinic Visits Needed
Day 14
Egg Retrieval Day
Day 33
Pregnancy Test Day

Phase 1 — Pre-Cycle Investigations (1–2 weeks before)

Before stimulation begins, both partners are fully investigated. These tests take 1–2 weeks and guide the personalised protocol chosen for you.

TimingEventWhat HappensYour Role
Week –2Initial ConsultationFull fertility history review; existing reports assessed; plan discussedAttend with partner; bring all previous fertility reports
Week –2Female Blood TestsAMH, Day 2 FSH/LH/E2, thyroid function, blood group, prolactin, Vitamin DBlood test on Day 2–3 of period for hormone tests
Week –2Baseline ScanAntral follicle count (AFC), uterine assessment, ovarian cyst checkAttend for transvaginal ultrasound
Week –2Semen AnalysisWHO 2021 semen parameters; morphology; DFI if indicatedPartner attends for semen analysis (3-day abstinence)
Week –1Protocol ReviewResults reviewed; stimulation protocol, doses, and calendar finalisedReview protocol with nurse coordinator; ask questions
Week –1Injection TrainingNurse demonstrates FSH pen/syringe technique; you practise with trainer penLearn injection technique; collect medications

Phase 2 — Stimulation Phase (Days 2–13)

The busiest phase — daily injections and regular scan appointments. Day numbering starts from Day 1 of your period.

Important: All injections should be given at the same time each day (e.g., 9pm every evening). Consistency of timing is important for optimal follicle development.
DayEventWhat HappensYour Role
Day 2🟢 Stimulation StartsBaseline scan confirms ovaries are quiet; first FSH (± LH) injection given at clinic or at homeAttend clinic for baseline scan; administer first injection as trained
Day 3Daily InjectionFSH (± LH) injection continuedSelf-administer at home at same time as Day 2
Day 4Daily InjectionFSH (± LH) injection continuedSelf-administer at home
Day 5–6📊 Monitoring Scan 1Transvaginal scan counts follicles and measures sizes (target: 10–12mm by Day 6–7); blood oestradiol (E2) level; dose adjusted if needed; GnRH antagonist addedAttend clinic for scan + blood test; start antagonist injection same evening
Day 6–7FSH + AntagonistBoth injections daily from now until trigger dayTwo separate subcutaneous injections each evening
Day 8📊 Monitoring Scan 2Follicle sizes 12–16mm; E2 and LH checked; dose adjusted; antagonist continuedAttend clinic for scan + blood test
Day 9FSH + AntagonistInjections continued as per adjusted doseSelf-administer at home
Day 10📊 Monitoring Scan 3Leading follicles 16–18mm; final dose check; trigger timing decidedAttend clinic; nurse may advise trigger the same evening or in 1–2 days
Day 11–12FSH + Antagonist (if not triggered)Some patients need 1–2 more days of stimulation if follicles not yet readyContinue injections until trigger day
Day 12–13⚡ Trigger InjectionWhen leading follicles reach 18–20mm, trigger (hCG or GnRH agonist) given at EXACTLY the prescribed hour (e.g., 10pm sharp); STOP all other injections; egg retrieval booked for 36 hours laterAdminister trigger at exact hour; confirm retrieval appointment; fast from midnight

Phase 3 — Egg Retrieval & Laboratory (Days 14–19)

DayEventWhat HappensYour Role
Day 14🥚 Egg Retrieval (OPU)IV sedation; transvaginal needle aspiration of all follicles (20–30 min); partner provides semen sample; embryologist identifies mature eggs; ICSI performed same afternoon; rest 2–3 hrs; discharged homeFast from midnight; arrive at clinic as instructed; partner attends; arrange lift home; rest remainder of day
Day 14 eveningStart ProgesteroneProgesterone pessaries or gel started the evening of retrieval (or next morning)Begin progesterone as prescribed; continue daily until told otherwise
Day 15📞 Fertilisation ReportEmbryologist calls with 2PN fertilisation count; embryos placed in incubatorsAwait phone call from embryologist; rest at home
Day 16Embryo Day 2 ReportEmbryologist may call with Day 2 cell counts (2–4 cell stage); no clinic visitContinue progesterone; rest
Day 17Embryo Day 3 Report8-cell stage check; embryologist may advise Day 3 transfer if few embryos remain, or continue to Day 5If Day 3 transfer advised: attend clinic; otherwise continue to Day 5
Day 18Morula StageEmbryos compacting into morula; Day 4 is generally quiet (no transfer)Continue progesterone; plan for Day 5 transfer appointment
Day 19🌱 Embryo TransferBest blastocyst(s) graded and selected; embryo transfer by thin catheter (5 min, painless, no anaesthesia); abdominal US guidance; rest 30 min; discharged; extra embryos vitrifiedDrink water before appointment; attend clinic; rest 30 min; go home; continue progesterone

Phase 4 — Two-Week Wait (Days 19–33)

The 14 days between embryo transfer and pregnancy test. Continue progesterone daily throughout.

Days Post-TransferWhat is Happening (Inside)What You May FeelYour Role
Days 1–2Embryo floating freely in uterine cavityMild cramping, bloating — from retrieval and progesteroneRest, gentle activity, continue progesterone
Days 3–4Blastocyst beginning to hatch from zona pellucidaProgesterone side effects (breast tenderness, bloating) commonNormal gentle activities; avoid heavy exercise
Days 5–7⭐ Implantation — embryo attaches to uterine liningSome women feel nothing; others notice mild spotting (implantation bleed) or mild cramping — all normalDo not test yet — too early; continue progesterone
Days 8–9hCG begins rising if implantation successfulVery early pregnancy symptoms may begin or may not — too variable to readResist urge to test — blood levels too low for home tests to reliably detect
Days 10–12hCG doubling every 48 hoursNausea, breast tenderness, fatigue may start — but symptoms don't predict resultHome test may show positive from Day 10–12, but false negatives and positives from progesterone occur — wait for blood test
Day 14 — Test Day🏆 Beta-hCG Blood TestBlood test gives definitive result; positive = pregnancy confirmed; result available same dayAttend clinic for blood test; await result call; do not stop progesterone until told to
Week 6–7 (if positive)Foetal heartbeat developingIncreasing pregnancy symptoms — nausea, fatigue, breast changesAttend 6–7 week scan to confirm heartbeat; transition to antenatal care
Important: Pregnancy symptoms during the 2WW are largely caused by progesterone — not pregnancy. Both positive and negative cycles have identical symptoms during the wait. The blood test is the only reliable indicator.
Dr. E. Prashanthi Reddy — IVF Specialist Hyderabad

Dr. E. Prashanthi Reddy

MBBS, DGO · ART Training — Kiel University, Germany · TGMC Reg: 50624
19+ Years Experience · 5,000+ ICSI Cycles · Mother Hospitals & IVF Center, Boduppal, Hyderabad
📞 97059 93366 / 97059 93355  |  💬 WhatsApp: 90520 74999

Frequently Asked Questions

How many days does an IVF cycle take from start to finish?
A typical IVF cycle from the first stimulation injection to the pregnancy test takes 28–33 days. Stimulation (injections) lasts 10–14 days, egg retrieval is on Day 14, embryo culture takes 5 days, and the pregnancy test is 14 days after embryo transfer. Pre-cycle investigations (blood tests, scans, semen analysis) add an additional 1–2 weeks before stimulation starts.
How many clinic visits are required during IVF?
During a stimulation cycle, expect 4–6 monitoring scan visits (approximately every 2–3 days, more frequent near the end of stimulation). Plus one visit for egg retrieval and one for embryo transfer. Pre-cycle investigations require 1–2 additional visits. Total: approximately 6–9 clinic visits per cycle. All visits are outpatient — no overnight admission required.
Can I work during IVF treatment?
Yes. Most women work normally throughout IVF. The stimulation phase requires early-morning or morning scan appointments every 2–3 days. Egg retrieval requires a full day off (sedation recovery). Embryo transfer requires a half-day. During the 2-week wait, normal work is encouraged. The main disruptions are the monitoring scan appointments and the retrieval/transfer days.
What day does the IVF cycle start?
The IVF cycle typically starts on Day 2 of your period (Day 1 = first day of full flow). A baseline scan is done on Day 2 to confirm ovaries are quiet before stimulation begins. You will be asked to call the clinic on Day 1 of your period to confirm the start date and book your Day 2 scan.

Explore the Full IVF Journey